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从苏黎世和巴塞尔两个地理区域来源的熊蜂宿主(Bombusterrestris)中分离了47个熊蜂短膜虫克隆。利用GPI、MDH、ME、6PGD和PGM五种等位基因酶电泳对这47个熊蜂短膜虫克隆的检测结果发现,熊蜂短膜虫克隆间缺乏理论基因型,说明熊蜂短膜虫的自然群体存在着克隆结构。两个地理群体的熊蜂短膜虫的克隆结构也具差别。根据等位基因酶推测熊蜂短膜虫的染色体为双倍体。利用脉冲场梯度凝胶电泳,清楚地分离了熊蜂短膜虫的九条染色体规模DNA带,其长度在不同地理群体的熊蜂短膜虫间都明显具有差别。实验证明,熊蜂短膜虫是由于地理区域宿主的隔离从古老的群体长期进化而来。
Forty-seven Bombus mosquitoes were isolated from the Bombusterrestris from two geographic regions of Zurich and Basel. The results showed that there was no theoretical genotype between the clones of Memphis Bombus, which indicated that the natural population of Memphis There is a cloning structure. There are also differences in the clonal structure of the Bombyx mandarinae between two geographical groups. According to the allelic enzyme speculated that the chromosome of the genus Memphis was diploid. By using pulsed-field gradient gel electrophoresis, nine chromosome-size DNA bands of Memphis discoidea were clearly separated, and their lengths differed markedly among different geographical groups of Bombus. Experiments show that the bumblebee is due to the geographical isolation of the host from the ancient group long-term evolution.