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目的研究肾癌患者手术前血清与对照人群血清的蛋白质差异及其临床意义。方法采用蛋白质芯片CM10技术鉴定40例肾癌患者(肾癌组)手术前血清与40例正常人(对照组)血清的蛋白质质谱的差异。结果两组质谱显示初步筛出质荷比在1 038、1 568、1 723、7 976、8 058、1 5 940、1 545、16 107、1 433、1 701、1 500共11个蛋白峰有明显差异,其敏感性为88.95%,特异性为83.68%。结论肾癌特异性蛋白质的发现,对进一步研究其发生机理及临床特异性标记物的发现有重要的意义。
Objective To study the protein difference of serum before and after operation in patients with renal cell carcinoma and its clinical significance. Methods The protein chip CM10 was used to identify the difference of protein mass spectrum between serum of 40 patients with renal cell carcinoma (RCC) before operation and 40 normal controls (control group). Results The mass spectra of the two groups showed that the mass-to-charge ratio was 11 038,1 568,1 723,7 976,8 058,1 5 940,1 545,16 107,1 433,1 701,1 500 There are significant differences, the sensitivity was 88.95%, specificity was 83.68%. Conclusion The discovery of renal cancer-specific proteins is of great significance for the further study of its pathogenesis and the discovery of clinical specific markers.