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目的 :体外观察白细胞介素 - 1(IL - 1)对白细胞介素 - 2 (IL - 2 )激活骨髓自然杀伤细胞 (NK )和淋巴因子 -激活杀伤细胞 (L AK)活性的影响及两者联合激活的骨髓对白血病细胞的净化作用。方法 :标准 4h5 1 Cr释放实验测定激活骨髓的细胞毒作用 ;集落培养法观察激活骨髓对 HL - 6 0和 K 5 6 2细胞的净化作用。结果 :10～ 2 0 0μg/ LIL- 1对正常骨髓的 NK和 L AK活性无明显影响 ;IL- 2能够增强骨髓的 NK活性和诱导 L AK细胞的产生 ,呈浓度递增趋势 ;10 0 μg/ L IL- 1能增强 1万～ 10 0万 U/ L IL- 2诱导的骨髓 NK及 L AK活性。在体外净化实验中 ,10 0μg/ L IL- 1或 10 0万 U/ L IL- 2对 K5 6 2和 (或 ) HL- 6 0细胞无直接杀伤 ,而在掺入 1% K5 6 2或 HL- 6 0的正常骨髓中 ,加入 IL- 1和 (或 ) IL- 2 ,对白血病细胞均有明显的毒性作用 ,以培养 3d的骨髓净化作用最强。IL- 2的净化效果优于 IL - 1(P <0 .0 5 )。两者联合激活的骨髓净化作用更为明显 ,1d和 3 d分别比单用 IL - 2增加 1～ 2个对数级 ,差异有显著性 (P <0 .0 5 )。 IL - 1和 (或 ) IL - 2对激活骨髓的 CFU- GM水平无明显影响。结论 :IL - 1能够明显增强IL - 2激活的骨髓对白血病细胞的净化作用。
OBJECTIVE: To observe the effects of interleukin - 1 (IL - 1) on the activities of natural killer (NK) and lymphokine activated killer (L AK) cells activated by interleukin - 2 (IL - 2) Purification of leukemic cells by co-activated bone marrow. METHODS: The standard 4h5 1 Cr release assay was used to determine the cytotoxicity of activated bone marrow. The colony culture was used to observe the decontamination of HL - 60 and K 5 6 2 cells by activated bone marrow. Results: 10 ~ 200 μg / L IL-1 had no effect on the activity of NK and L AK in normal bone marrow. IL-2 increased the activity of NK and induced the proliferation of L AK cells in bone marrow. L IL-1 enhanced NK and L AK activity induced by IL-2 between 10 000 and 100 000 U / L. In in vitro purification experiments, K562 and / or HL-60 cells were not directly killed by 100 μg / L IL-1 or 10 million U / L IL-2, HL-60 in normal bone marrow, adding IL-1 and / or IL-2, have obvious toxic effects on leukemia cells in order to cultivate the strongest bone marrow purification effect of 3d. The purification effect of IL-2 was better than IL-1 (P <0.05). The combination of the two activated the myeloid decontamination even more markedly, with a 1 to 2 logarithmic increase on day 1 and day 3, respectively, with a significant difference (P <0.05). IL - 1 and / or IL - 2 had no significant effect on the activation of bone marrow CFU - GM. Conclusion: IL - 1 can obviously enhance the purifying effect of IL - 2 activated bone marrow on leukemia cells.