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为探究LEPR基因的遗传多态性,丰富山羊LEPR基因的研究,本研究以贵州黑山羊和黔北麻羊为试验材料,运用DNA池结合直接测序方法进行LEPR基因SNPs位点的筛选,从而对突变的SNPs位点进行RNA二级结构以及所编码蛋白质的二级结构和三级结构进行生物信息学分析。在试羊LEPR基因中共发现4个SNPs,分别为Exon7-T81C(同义突变)、Exon8-C39T(同义突变)、Exon10-A70G(Asn-Ser)和Exon18-C94T(Ser-Leu)。分析表明,4个位点突变前后的等位基因频率、mRNA二级结构的最小自由能、LEPR蛋白质二级结构和三级结构均有改变。结果表明,LEPR基因拥有较为丰富的遗传多态性。
In order to explore genetic polymorphism of LEPR gene and enrich LEPR gene in goats, this study took Guizhou black goat and Qianbei mutton as experimental materials, and used DNA pool combined with direct sequencing method to screen SNPs of LEPR gene. The SNPs were mutated to carry out bioinformatic analysis of RNA secondary structure and the secondary structure and tertiary structure of the encoded protein. Four SNPs were found in the test sheep LEPR gene, including Exon7-T81C (synonymous mutation), Exon8-C39T (synonymous mutation), Exon10-A70G (Asn-Ser) and Exon18-C94T (Ser-Leu). The results showed that allele frequency, minimum free energy of mRNA secondary structure, LEPR secondary structure and tertiary structure of four loci were changed before and after mutation. The results showed that LEPR gene has a rich genetic polymorphism.