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目的 :探讨亚硒酸钠对大鼠胃癌变前期胃窦粘膜 G细胞、D细胞和 EC细胞的影响。方法 :用免疫组织化学及图像分析法 ,观察亚硒酸钠对 N-甲基 - N’-硝基 - N-亚硝基胍诱发的大鼠胃癌变前期胃窦粘膜 G细胞、D细胞和 EC细胞的变化。结果 :N-甲基 - N’-硝基 - N-亚硝基胍短期灌胃后 (阳性对照组 ) ,胃窦粘膜 G、D、EC细胞的数量和平均光密度值 (OD值 )与正常对照组比较均显著减少 (P<0 .0 1)。 N-甲基 - N’-硝基 - N-亚硝基胍灌胃后给含亚硒酸钠的硒饲料 ,低剂量硒组 G细胞数量与阳性对照组比较无明显变化 (P>0 .0 5) ,但 OD值明显增高 (P<0 .0 1) ;高剂量硒组 G细胞数量及 OD值均显著高于阳性对照组 (P<0 .0 5,P<0 .0 1) ;低硒组和高硒组 EC细胞数及 OD值与阳性对照组比较 ,均有显著性差异 (P<0 .0 5,P<0 .0 1) ;D细胞与阳性对照组比较无变化。结论 :胃窦粘膜 G细胞、EC细胞及其分泌的生物活性物质可能参与了硒抗大鼠胃癌变前期的调控作用
Objective: To investigate the effects of sodium selenite on G cells, D cells and EC cells in gastric antrum of anterior gastric mucosa in rats. Methods: Immunohistochemistry and image analysis were used to observe the effect of sodium selenite on the changes of G cells, D cells and gastric mucosa in gastric antrum of rats induced by N-methyl-N’-nitro-N-nitrosoguanidine EC cell changes. Results: The numbers and average optical density (OD) values of G, D and EC cells in antral mucosa after N-methyl-N’-nitro-N-nitrosoguanidine administration were The normal control group were significantly reduced (P <0.01). After seeding with sodium selenite, the number of G cells in low dose selenium group had no significant change compared with the positive control group (P> 0.05) after N - methyl - N ’- nitro - N - (P <0.01). The number of G cells and OD in high-dose selenium group were significantly higher than those in positive control group (P <0.05, P <0.01) ; The number of EC cells and OD value in low selenium group and high selenium group were significantly different from those in positive control group (P <0.05, P <0.01); there was no change in D cells compared with positive control group . CONCLUSIONS: Antral mucosal G cells, EC cells and their secreted biologically active substances may be involved in the regulation of selenium against gastric precancerous lesions in rats