论文部分内容阅读
对籼稻材料PT46(高脂肪酶活性)和WP20(低脂肪酶活性)及其F2群体进行了脂肪酶活性的定量测定和遗传分析。χ2测验结果表明,F2群体表现型分布符合1∶2∶1的孟德尔分离比,表明脂肪酶活性高低由1对单基因控制,低脂肪酶活性为隐性性状。结合SSR分子标记,以F2群体为定位群体,将脂肪酶活性基因定位在水稻第3染色体上,与SSR标记RM7和RM232之间的遗传距离分别为14.8cM和4.1cM,暂命名为la(lipase activity)。采用人工老化的方法,以种子发芽率和老化指数评价两亲本及其F2群体的耐储藏特性,结合所测定的脂肪酶活性数据进行相关性分析。结果表明,随着老化时间的延长,脂肪酶活性高的材料,发芽率降低迅速,老化指数增加很快,而脂肪酶活性较低的材料,老化指数变化相对较慢,即脂肪酶活性低的材料较耐储藏。经10、20d人工加速老化处理后的种子老化指数与种胚脂肪酶活性的相关系数分别为0.6165**和0.4703**,表明老化指数与脂肪酶活性呈显著正相关。
The quantitative analysis and genetic analysis of the lipase activity of PT46 (high lipase activity) and WP20 (low lipase activity) and their F2 population of indica rice were carried out. The results of χ2 test showed that phenotypic distribution of F2 population corresponded to Mendelian segregation ratio of 1: 2: 1, indicating that the activity of lipase was controlled by a single gene and the activity of low lipase was recessive. Combined with SSR markers, F2 locus was used as the locating group. The lipase activity gene was mapped on chromosome 3 of rice with genetic distances of 14.8cM and 4.1cM from those of SSR markers RM7 and RM232, respectively, and temporarily named as la (lipase activity). The artificial aging method was used to evaluate the storage resistance of the parents and their F2 population with the seed germination rate and aging index. The correlation analysis was carried out based on the measured lipase activity data. The results showed that the material with high lipase activity decreased rapidly and the aging index increased rapidly with the aging time prolonged, while the material with lower lipase activity changed relatively slowly, that is, the lipase activity was low Material is more resistant to storage. The correlation coefficient between seed aging index and seed embryo lipase activity after artificial aging accelerated 10 and 20 days were 0.6165 ** and 0.4703 **, respectively, indicating that there was a significant positive correlation between aging index and lipase activity.