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以自旋标记物Ｔｅｍｐｏ（２，２，６，６－四甲基哌啶－１－氮氧基）标记杨树叶膜磷脂酰甘油（ＰＧ），通过电子自旋共振波谱法（ＥＳＲ）测定了杨树不同抗寒品种及其杂种后代ＰＧ的相变温度。同时用气相色谱法对ＰＧ脂肪酸组成进行了分析。结果表明，不耐寒的美洲黑杨（Ｐｏｐｕｌｕｓｄｅｌｔｏｉｄｅｓｃｖ＇Ｌｕｘ＇Ｉ６９／５５母本）的ＰＧ在４℃左右发生相变，抗寒的欧美杨（ＰｏｐｕｌｕｓｅｕｒａｍｅｒｉｃａｎａｃｖＩ－４５／５１，父本）的ＰＧ则在－１２℃发生相变，６个杂种后代均在－２℃－１１℃范围内发生相变。而且磷脂的脂肪酸组成分析表明，不同抗寒品种叶内ＰＧ的饱和脂肪酸水与ＰＧ的热致相变行为相关。抗寒品种的ＰＧ饱和脂肪酸水平低于不抗寒品种，以致前者的ＰＧ相变温度低于后者从而表现为抗寒。
Poplar leaf membrane phosphatidyl glycerol (PG) was labeled with the spin label Tempo (2,2,6,6-tetramethylpiperidine-1-oxyloxy) and determined by electron spin resonance spectroscopy (ESR) Phase Transition Temperatures of Different Winter Rape Varieties and Their Hybrid Offspring. The composition of PG fatty acids was also analyzed by gas chromatography. The results showed that the PG of Populus deltoides cv’Lux’I69 / 55 female parent did not change phase at about 4 ℃. The PG of Populus deltoi cv’acvI-45/51, The phase transition occurred at 12 ℃, and all the 6 hybrid offspring were in the range of -2 ℃ -11 ℃. Moreover, the fatty acid composition analysis of phospholipids showed that the saturated fatty acid water of PG in leaves of different cold-resistant varieties was related to the thermal induced phase transformation behavior of PG. Cold-resistant varieties of PG saturated fatty acid levels are lower than the non-cold-resistant varieties, so that the former PG phase transition temperature is lower than the latter to show the cold.