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目的在动物水平研究超声联合微泡增效骨髓干细胞(BMSCs)心肌移植的作用,为临床上提高心肌梗死后BMSCs移植效率提供一种新的思路。方法12只中国家猪分别体外分离纯化BMSCs,超顺磁性氧化铁颗粒标记;介入法建立急性心肌梗死模型,成功后14d经冠状动脉注入自体BMSCs;实验组先注入微泡后再注入BMSCs,同时行超声辐射。术后行磁共振检查;心肌做病理切光镜、透射电镜观察。结果①两组在心肌梗死区和梗死周边区磁共振上均见SPIO标记的BMSCs形成的低密度区;②实验组较对照组普鲁士蓝染色阳性细胞明显增多(P<0.01);③两组心肌超微结构无差异,但B1组见血管内皮间隙增宽。结论超声辐射微泡可增加自体BMSCs心脏的移植效率。
OBJECTIVE: To study the effect of ultrasound combined with microbubble-augmented bone marrow stem cells (BMSCs) on myocardial transplantation in animals and to provide a new idea for improving the transplantation efficiency of BMSCs after myocardial infarction. Methods BMSCs and superparamagnetic iron oxide (PMMA) particles were isolated and purified from 12 Chinese domestic pigs respectively. Acute myocardial infarction (AMI) model was established by intervention and injected with autologous BMSCs via coronary artery 14 days after successful injection. In the experimental group, BMSCs were injected into the microvesicles and injected with BMSCs Line ultrasound radiation. Postoperative magnetic resonance imaging; myocardial pathology light microscopy, transmission electron microscopy. Results ① The density of the SPIO-labeled BMSCs in the myocardial infarction area and the peripheral infarction area was lower than that in the control group (P <0.01). ③ The myocardial cells in the experimental group were significantly increased compared with the control group (P <0.01) There was no difference in ultrastructure, but in group B1, the vascular endothelial gap widened. Conclusion Ultrasound radiation microbubbles can increase the transplanted efficiency of autologous BMSCs.