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天疱疮(PV)诊断方法中,活检、免疫荧光和传统的涂片检查Tzanck细胞等方法各存在一些缺点。本文作者用一种免疫组化方法进行涂片细胞学分析,试图在同一标本上同时获得免疫细胞化学染色和形态学资料。 材料和方法18个人参与此项研究,10名通过涂片和活检确诊为PV,4名为疱疹性口炎,4名为健康人。本研究应用了以下4种方法:①组织切片进行免疫荧光染色;②巴氏细胞涂片染色;③碱性磷酸酶—抗碱性磷酸酶(APAAP)技术进行涂片免疫染色,第一抗体
Pemphigus (PV) diagnostic methods, biopsy, immunofluorescence and traditional smear Tzanck cells and other methods, there are some shortcomings. The authors used an immunohistochemical smear cytology assay to try to obtain both immunocytochemical staining and morphological data on the same specimen. MATERIALS AND METHODS Eighteen individuals participated in the study, 10 were diagnosed with PV by smear and biopsy, 4 were herpetic stomatitis, and 4 were healthy. In this study, the following four methods were applied: ①Immunofluorescence staining of tissue sections; ② Pap smear staining; ③Alkaline phosphatase-alkaline phosphatase (APAAP) technique for smear immunostaining, the primary antibody