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目的:建立浊点萃取高效液相色谱法测定秦皮中秦皮甲素和秦皮乙素含量的方法。方法:选择以表面活性剂Genapol X-080作为萃取剂,考察了表面活性剂浓度、液固比、盐浓度、平衡温度、平衡时间等因素对萃取结果的影响。结果:获得最佳萃取条件为Genapol X-080的质量浓度为0.18 g·m L-1,液固比定为100∶1,盐浓度1.5 mol·L-1,平衡温度60℃,平衡时间35 min。结论:浊点萃取是一种安全、高效、简便的样品处理方法。在最佳条件下,秦皮甲素和秦皮乙素的加样回收率分别为95.3%,96.0%,RSD分别为2.3%,2.1%。利用此方法成功测定了秦皮中秦皮甲素和秦皮乙素的含量。
Objective: To establish a method for the determination of aesculin and aesculetin in Qindian Peel by high performance liquid chromatography with cloud point extraction. Methods: The surfactant Genapol X-080 was selected as the extractant to investigate the effects of surfactant concentration, liquid-solid ratio, salt concentration, equilibrium temperature and equilibrium time on extraction results. Results: The optimal extraction conditions for Genapol X-080 were as follows: mass concentration 0.18 g · mL -1, liquid-solid ratio 100:1, salt concentration 1.5 mol·L -1, equilibrium temperature 60 ℃, equilibrium time 35 min. Conclusion: Cloud point extraction is a safe, efficient and simple sample processing method. Under the optimal conditions, the recoveries of aesculin and aesculetin were 95.3% and 96.0%, respectively, with RSDs of 2.3% and 2.1%, respectively. Using this method, the contents of aesculin and aesculetin in Cortex Nigra were determined successfully.