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发明一种组织微阵列供体取样与受体蜡块制作的新器具和新方法.利用这种新器具和新方法成功地制作了分别含448和390个供体组织点阵的组织微阵列受体蜡块和切片.这种新方法制作的组织微阵列切片经H&E染色,显微镜下观察证实,所有切片均无供体点阵组织脱落,切片厚度适中,组织结构无挤压变形,细胞形态均匀一致.免疫组化检测P53和P16蛋白在组织微阵列切片与其相应的常规组织切片中的表达结果完全一致.这种组织微阵列供体取样与受体蜡块制作新器具和新方法成本低廉,操作简便,具有在实验室推广应用的价值.
The invention discloses a new device and a new method for tissue microarray donor sampling and acceptor wax block making, and the tissue microarray with 448 and 390 donor tissue lattice respectively is successfully made by using the new apparatus and the new method. Body wax block and section.The tissue microarray sections made by this new method were stained by H & E and observed under a microscope. None of the sections had any shedding of the dot matrix, moderate slice thickness, no extrusion deformation of the tissue structure, uniform cell morphology Consistent with the immunohistochemical detection of P53 and P16 protein in tissue microarray sections and its corresponding conventional tissue sections of the expression of the results of this tissue microarray donor sample and acceptor wax block new device and a new method of low cost, Easy to operate, with the value of promoting the application in the laboratory.