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以Ｆｕｒａ２／ＡＭ为细胞内钙离子的荧光指示剂，用ＡＲＣＭＭＩＣ阳离子测定系统，直接测定了体外培养的新生大鼠神经细胞内游离钙（［Ｃａ２＋］ｉ）值，并观察了小檗碱（Ｂｅｒ）的影响。结果表明，Ｂｅｒ对神经细胞静息［Ｃａ２＋］ｉ无明显影响，Ｂｅｒ１～１００μｍｏｌ·Ｌ－１能剂量依赖地抑制去甲肾上腺素和Ｈ２Ｏ２引起的［Ｃａ２＋］ｉ升高，其ＩＣ５０分别为３９．９和１７．９μｍｏｌ·Ｌ－１。高剂量Ｂｅｒ（１０～１００μｍｏｌ·Ｌ－１）能抑制高Ｋ＋引起的［Ｃａ２＋］ｉ升高。姐果提示，Ｂｅｒ对去甲肾上腺素，高Ｋ＋及Ｈ２Ｏ２引起的［Ｃａ２＋］ｉ升高的抑制作用可能是其抗脑缺血作用机制之一。
Fura 2 / AM as a fluorescent indicator of intracellular calcium with ARCMICM cation determination system, directly measured in vitro cultured neonatal rat intracellular free calcium ([Ca2] i) value and observed The effect of berberine (Ber). The results showed that Ber had no effect on resting [Ca2 +] i in neurons. Ber1 ~ 100μmol·L-1 dose-dependently inhibited the increase of [Ca2 +] i induced by norepinephrine and H2O2 with IC50 of 39. 9 and 17.9μmol·L-1. High doses of Ber (10 ~ 100μmol·L-1) inhibited the increase of [Ca2 +] i induced by high K +. Sister fruit suggest that Ber on norepinephrine, high K + and H2O2-induced [Ca2 +] i inhibition may be one of its anti-cerebral ischemia mechanism.