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合成了一种轴向尼泊金甲酯取代的酞菁硅,即二(4-甲酯基苯氧基)酞菁硅,并通过IR,NMR,HPLC和元素分析等手段进行了表征。研究了该配合物在不同溶剂中的电子吸收光谱和荧光光谱。结果表明,它在含2%Cremophor EL,20%丙二醇的生理盐水中,以单体形式存在,Q带最大吸收位于683 nm附近,相应的摩尔吸光系数为7.47×104mol-1.L.cm-1,最大发射带位于690 nm附近,荧光量子产率为0.34,荧光寿命为4.7 ns。初步的离体光动力活性测试表明,该配合物对B16黑色素瘤细胞具有光动力灭活能力,半致死量LD50为1.2×10-4mol.L-1。
An axially-oriented methylparaben-substituted phthalocyanine, bis (4-carbomethoxy) phthalocyanine, was synthesized and characterized by means of IR, NMR, HPLC and elemental analysis. The electron absorption and fluorescence spectra of the complex in different solvents were studied. The results showed that it exists as a monomer in physiological saline containing 2% Cremophor EL and 20% propylene glycol with the maximum Q band absorption at 683 nm and the corresponding molar extinction coefficient of 7.47 × 104mol-1.L.cm- 1, the maximum emission band is located near 690 nm, the fluorescence quantum yield is 0.34, and the fluorescence lifetime is 4.7 ns. Preliminary in vitro photodynamic activity tests showed that the complex has photodynamic inactivation of B16 melanoma cells, with a LD50 of 1.2 × 10 -4 mol·L -1.