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目的 探讨应用平阳霉素短程化疗对在体喉鳞癌细胞超微结构的影响，以论证临床应用该方法的科学性和合理性。方法 随机选择６例经病理确诊的喉鳞状细胞癌患者，化疗前后分别取活组织行透射电镜观察。结果 化疗后肿瘤细胞的超微结构出现明显变化。表现为细胞核及核膜变形，核仁碎裂、崩解、固缩或消失，细胞质内线粒体明显减少，嵴结构模糊不清，出现空泡变性，核内染色质变性呈不均匀粗块状，但桥粒结构和张力原纤维无明显变化。结论 平阳霉素能使喉鳞癌细胞的超微结构产生明显改变，从而可有效地抑制肿瘤细胞增殖，术前应用平阳霉素可杀灭微小病灶，提高手术切除率。
Objective To investigate the effect of pingyangmycin short-term chemotherapy on the ultrastructure of laryngeal squamous cell carcinoma (LSCC) in vivo and to demonstrate the scientific and rationality of this method in clinical application. Methods Six patients with pathologically confirmed laryngeal squamous cell carcinoma were randomly selected. The living tissues were collected before and after chemotherapy for transmission electron microscopy. Results After chemotherapy, the ultrastructure of tumor cells showed obvious changes. Manifested as cell nucleus and nuclear membrane deformation, disintegration of nucleolus, disintegration, shrinkage or disappearance of mitochondria significantly reduced cytoplasm, ridge structure blurred, vacuolar degeneration, nuclear chromatin degeneration was heterogeneous rough block, But the desmosomal structure and tension fibril no significant change. Conclusion Pingyangmycin can significantly change the ultrastructure of laryngeal squamous cell carcinoma, which can effectively inhibit the proliferation of tumor cells. Preoperative application of pingyangmycin can kill tiny lesions and improve the surgical resection rate.