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菜用甘薯抗寒性能是其在早春及秋冬季作为蔬菜供应的重要制约因素。以徐菜薯1号菜用甘薯为实验材料,通过对甘薯叶进行外源2,4-表油菜素内酯(EBR)喷施处理,探究其对菜用甘薯抗寒生理生化的影响;并对其诱导抗寒的机理进行研究。结果表明,0.1 mg·L~(-1)的EBR处理下甘薯的抗寒性能最佳。与对照相比在8℃低温胁迫下的48 h内,甘薯叶中3种抗氧化酶(CAT、POD和SOD)活性明显高于对照,3种渗透调节物质(可溶性蛋白质、可溶性糖和脯氨酸)中可溶性蛋白的含量有大幅提高,而细胞的损伤指标(电导率和MDA)的数值均低于对照。外源EBR处理提高了甘薯叶中抗氧化酶的活性及渗透调节物质的含量,减少了细胞膜的损伤,增强了菜用甘薯抵抗低温胁迫的能力。
Cold hardiness of vegetable sweet potato is an important restraining factor of vegetable supply in early spring, autumn and winter. In order to explore the effect of exogenous 2,4-epibrassinolide (EBR) on sweet potato leaf physiology and biochemistry in sweet potato, The mechanism of induced cold resistance was studied. The results showed that sweetpotato had the best cold resistance under the treatment of 0.1 mg · L -1 EBR. Compared with the control, the activities of three antioxidant enzymes (CAT, POD and SOD) in sweet potato leaves were significantly higher than those in the control within 48 h under the low temperature stress of 8 ℃. The three osmotic adjustment substances (soluble protein, soluble sugar and proline Acid) soluble protein content increased significantly, while the cell damage indicators (conductivity and MDA) values ?? were lower than the control. Exogenous EBR treatment increased the activity of antioxidant enzymes and osmotic adjustment substances in sweet potato leaves, reduced the damage of cell membrane and enhanced the ability of sweetpotato to resist the cold stress.