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利用示踪剂FLNa在脑缺血及再灌注的动物模型上 ,通过活体观察和测定血液、脑等脏器的荧光强度 ,以及对软脑膜微血管荧光图象的平滑处理与定量分析,研究软脑膜微血管的通透性 ,探讨脑缺血及再灌注对微血管通透性的影响及内在规律。实验结果表明 :缺血、缺血及再灌注会引起微血管内皮细胞的损伤 ,导致微血管通透性增大 ,这种损伤一般发生在缺血或再灌注早期 ,虽然各脏器微血管都受到损伤 ,但其荧光值不同 ,说明各脏器抗缺血与缺氧的能力不同 ,其中脑与脾最强 ,肝、肾最弱 ,而肺的测量值比较特殊 ,这一特殊性与肺的自身结构有关 ;长时间的血液再灌注对损伤有修复作用。此外 ,还提出了图象处理与分析的方法 ,建立了荧光物质在微血管外的扩散方程 ,并求出了扩散速度。
The use of tracer FLNa in cerebral ischemia and reperfusion animal model by in vivo observation and determination of blood, brain and other organs of the fluorescence intensity, and the smooth plexus of the microvascular fluorescence image processing and quantitative analysis of pia mater Microvascular permeability, to explore the impact of cerebral ischemia and reperfusion on microvascular permeability and the inherent law. The experimental results show that: ischemia, ischemia and reperfusion can cause damage to microvascular endothelial cells, resulting in increased permeability of microvessels. This injury usually occurs in the early stage of ischemia or reperfusion. Although the microvessels of the organs are damaged, However, their fluorescence values are different, indicating that each organ has different ability to resist ischemia and hypoxia. Among them, the strongest brain and spleen, the weakest liver and kidney, and the measured value of lung are rather special. This particularity is related to its own structure Related; prolonged blood reperfusion injury repair. In addition, the method of image processing and analysis is also proposed, the diffusion equation of fluorescent substance outside the microvessel is established, and the diffusion speed is obtained.