目的:评价程序性坏死在自体原位肝移植术大鼠肺损伤中的作用。方法:清洁级健康成年雄性SD大鼠24只，体重250～280 g，10～12周龄，采用随机数字表法分为3组(n n＝8)：假手术组(S组)、自体原位肝移植术组(T组)和程序性坏死抑制剂Necrostatin-1组(N组)。S组仅单纯进行开腹，游离相应的血管和韧带，最后关腹；T组和N组均制备自体原位肝移植术大鼠肺损伤模型，N组术前30 min腹腔注射Necrostatin-1 1 mg/kg。于再灌注6 h时处死大鼠取肺组织，计算肺组织湿/干重(W/D)比值，采用ELISA法测定TNF-α和IL-6含量，采用免疫组化法测定混合系列蛋白酶样结构域(MLKL)表达，采用Western blot法检测受体相互作用蛋白激酶1(RIPK1)和受体相互作用蛋白激酶3(RIPK3)的表达。n 结果:与S组比较，T组和N组肺组织W/D比值、TNF-α和IL-6含量升高；RIPK1、RIPK3和MLKL表达上调(n P<0.05)；与T组比较，N组肺组织W/D比值、TNF-α和IL-6含量降低，肺组织RIPK1、RIPK3和MLKL表达下调(n P<0.05)。S组肺组织形态未见明显异常，N组肺组织病理学损伤较T组明显减轻。n 结论:程序性坏死参与了自体原位肝移植术大鼠肺损伤的过程。“,”Objective:To evaluate the role of necroptosis in lung injury in a rat model of autologous orthotopic liver transplantation.Methods:Twenty-four clean-grade healthy adult male Sprague-Dawley rats, weighing 250-280 g, aged 10-12 weeks, were divided into 3 groups (n n=8 each) by a random number table method: sham operation group (S group), autologous orthotopic liver transplantation group (T group) and necroptosis inhibitor necrostatin-1 group (N group). In S group, the abdomen was opened, the corresponding blood vessels and ligaments were isolated, and the abdomen was finally closed.The lung injury models were established after autologous orthotopic liver transplantation in T and N groups, and necrostatin-1 1 mg/kg was intraperitoneally injected at 30 min before surgery in N group.Rats were sacrificed at 6 h of reperfusion, and lung tissues were removed for determination of the wet/dry weight ratio (W/D ratio), contents of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) (by enzyme-linked immunosorbent assay), expression of mixed lineage kinase domain-like protein (MLKL) (by immunohistochemistry), and expression of receptor-interacting protein kinase 1 (RIPK1) and RIPK3 (by Western blot).n Results:Compared with S group, the W/D ratio and contents of TNF-α and IL-6 were significantly increased, and the expression of RIPK1, RIPK3 and MLKL was up-regulated in T and N groups (n P<0.05). Compared with T group, the W/D ratio and contents of TNF-α and IL-6 were significantly decreased, and the expression of RIPK1, RIPK3 and MLKL was down-regulated in N group (n P<0.05). No obvious abnormality was found in the morphology of lung tissues in S group, and the pathological changes were significantly attenuated in N group when compared with T group.n Conclusion:Necroptosis is involved in the process of lung injury in a rat model of autologous orthotopic liver transplantation.