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目的了解云南省昆明地区高危人群艾滋病病毒Ⅰ型(HIV-1)二重感染的发生情况与特征。方法筛选昆明地区高危人群出现HIV-1二重感染的病例。通过不同的PCR/测序引物得到不同的测序结果,或直接测序时碱基模糊不清而确定的可疑二重感染病例,然后采用TA克隆的方法对单个PCR克隆进行测序,找到血浆标本中的HIV-1准种,并分析其系统发生和重组结构。结果昆明地区的高危人群中,HIV-1二重感染的发生率为7.7%(2/26)。2名具有高危异性性接触史的HIV-1感染者,分别为CRF01_AE和CRF08_BC或CRF07_BC的二重感染。结论昆明地区高危人群HIV-1的二重感染较为常见,这为快速产生新的重组病毒株提供了基础。该研究提示,该地区高危人群经常暴露于新的病毒株,将加速基因型内重组的发生。
Objective To investigate the occurrence and characteristics of HIV-1 double infection in high-risk population in Kunming, Yunnan Province. Methods Screening cases of HIV-1 double infection in high-risk population in Kunming area. Different sequencing results were obtained by different PCR / sequencing primers, or suspicious double infection cases were identified by direct blurry sequencing. Single PCR cloning was sequenced by TA cloning method to find HIV in plasma samples -1 quasispecies and analyze their phylogeny and recombination structures. Results The incidence of HIV-1 double infection was 7.7% (2/26) in high-risk population in Kunming area. Two HIV-1 infected individuals with a history of high-risk heterosexual exposure were a double infection of CRF01_AE and CRF08_BC or CRF07_BC, respectively. Conclusions Double infection of HIV-1 in high-risk population in Kunming area is more common, which provides a basis for rapid generation of new recombinant virus strains. The study suggests that frequent exposure of high-risk groups in the region to new strains will accelerate genotypic recombination.