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目的:研究胃溃疡对大鼠G细胞分泌胃泌素(D细胞分泌生长抑素)和对G(D)细胞变化的影响.方法:建立冰乙酸性大鼠胃溃疡模型,采用大体、光镜、透射电镜观察胃窦黏膜组织学表现和胃窦黏膜细胞超微结构表现,采用放射免疫法检测血清和胃窦组织中的胃泌素、生长抑素含量,采用免疫组化法及定量分析检测G(D)细胞形态、数目、面积、和G/D细胞数目、面积比值,采用免疫电镜及定量分析观察与检测G(D)细胞及G(D)细胞中的胃泌素(生长抑素)分泌颗粒.结果:免疫电镜观察到了G(D)细胞和IG(D)细胞中的胃泌素(生长抑素)分泌颗粒.大鼠胃溃疡后,G细胞内胃泌素分泌量增加,D细胞内生长抑素分泌量减少,G细胞数目增加、面积减少,D细胞数目减少、面积减少,G/D细胞数目比值和G/D细胞面积比值增加,并且血清、胃窦组织中胃泌素含量增加,生长抑素含量减少.结论:大鼠胃溃疡可引起G、D细胞的变化,引起G、D细胞分泌胃泌素、生长抑素的变化,和引起胃泌素、生长抑素含量的变化.
Objective: To study the effect of gastric ulcer on the secretion of somatostatin from G cells and the change of G (D) cells.Methods: The model of gastric ulcer in rats with glacial acetic acid was established, The histopathological changes of gastric mucosa and ultrastructure of gastric mucosa were observed by transmission electron microscopy. The contents of gastrin and somatostatin in serum and gastric antrum were detected by radioimmunoassay. Immunohistochemistry and quantitative analysis G (D) cell morphology, number, area, and G / D cell number and area ratio were observed by Giemsa staining and immunohistochemistry. The levels of gastrin in G (D) ) Secreted granules.Results: Gastrin (somatostatin) secreting granules were observed in G (D) cells and IG (D) cells by immunoelectron microscopy.Gastrin secretion increased in G cells after gastric ulcer in rats, The amount of somatostatin in D cells decreased, the number of G cells increased, the area decreased, the number of D cells decreased, the area decreased, the ratio of G / D cell number and G / D cell area increased, Superoxide dismutase increased, but somatostatin decreased.Conclusion: Gastric ulcer can cause gastric ulcer in rats Changes D cells, causing G, D cells secrete gastrin somatostatin, gastrin and cause changes, somatostatin content.