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目的探讨氦氖激光对缺血缺氧性脑损伤(HIBD)新生大鼠脑认知功能的影响及可能机制。方法将Wistar新生大鼠随机分为假手术组、模型组和激光干预组。结扎新生大鼠左颈总动脉后,再吸入低浓度氧制备HIBD动物模型。Y-型迷宫检测大鼠学习记忆能力。免疫组织化学方法检测海马内Nestin和胆碱乙酰转移酶(choline acetyl transferase,ChAT)的表达。结果氦氖激光干预后HIBD大鼠学习记忆能力明显改善(P<0.05),与模型组相比,激光干预组海马区Nestin和ChAT的表达明显升高(P<0.05)。结论氦氖激光明显提高HIBD新生大鼠脑认知功能,机制可能与激光促进HIBD大鼠内源性神经干细胞增殖以及脑组织合成神经递质乙酰胆碱增多,进而提高缺血缺氧后神经细胞数量和功能有关。
Objective To investigate the effects of He-Ne laser on cognitive function in neonatal rats with hypoxic-ischemic brain damage (HIBD) and its possible mechanism. Methods Wistar newborn rats were randomly divided into sham operation group, model group and laser intervention group. Ligation of neonatal rats left common carotid artery, and then inhaled low concentrations of oxygen prepared HIBD animal model. Y-type maze test learning and memory ability of rats. The expression of Nestin and choline acetyl transferase (ChAT) in the hippocampus were detected by immunohistochemistry. Results Compared with model group, the expression of Nestin and ChAT in hippocampus of laser-treated group was significantly increased (P <0.05). Conclusion He-Ne laser can significantly improve brain cognitive function in neonatal rats with HIBD. The mechanism may be related to the laser-induced proliferation of endogenous neural stem cells and the increase of acetylcholine, a neurotransmitter in HIBD rats, Function related.