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虽然已经有大豆下胚轴再生植株的很多报道,但再生率总是很低并且不定芽的再生都是在添加细胞分裂素的培养基上取得的。这种培养基使外植体形成大量愈伤组织且阻碍了再生芽的进一步生长。为此本实验将表面灭菌的成熟种子置于2种培养基上萌发,一种添加2 mg/L苄基腺嘌呤(BA)而另一种不添加;6 d后将小苗的下胚轴切下作为外植体培养到两种诱导不定芽再生的培养基上,同样一种添加2 mg/L BA而另一种不添加。在9个当地、2个美国和1个巴西共12个供试品系中,最好的培养结果是在含2 mg/L BA的培养基上萌发、长苗、切取下胚轴并在不含BA的再生培养基诱导不定芽。以此程序,不定芽的再生率和质量都得到了显著提高,其中一个当地品种“广大粒”的再生率达到了72%。
Although there have been many reports of regenerated plants of soybean hypocotyls, the regeneration rate is always low and the regeneration of adventitious buds is obtained on the medium supplemented with cytokinin. This medium causes explants to form large numbers of calluses and hinders further growth of regenerated shoots. In this experiment, the surface-sterilized mature seeds were germinated on two kinds of medium, one was added with 2 mg / L benzyl adenine (BA) and the other was not added. After 6 days, the seedling hypocotyl Cut off as explants and cultured on two mediums that induce regeneration of adventitious buds, one with 2 mg / L BA and the other without addition. Among 12 tested lines from 9 local, 2 U.S. and 1 Brazil, the best culture results were germination, long shoots on hypocotyls containing 2 mg / L BA, hypocotyl excised, BA regeneration medium induced adventitious buds. With this procedure, the regeneration rate and quality of adventitious buds have been significantly improved, of which the regeneration rate of a local variety “granule” reached 72%.