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探讨黄芪皂苷对化疗贫血小鼠PI3K/Akt/mTOR相关基因mRNA表达的影响。选取6~7周龄BALB/c小鼠48只,雄性,随机分为空白组、模型组、皂苷组、甲苷组,每组12只。采用环磷酰胺建立化疗贫血模型,灌胃治疗14 d后,摘眼球取血,QPCR法检测脾脏中Akt,PI3K,BCL-xl,bad,Fox O,mTOR,PTEN的mRNA水平。结果表明,模型组血红细胞计数,血红蛋白含量与空白组、皂苷组和甲苷组比较,明显降低(P<0.05)。与空白组、皂苷组、甲苷组相比,模型组Akt,PI3K,BCL-xl,bad,mTOR水平明显降低(P<0.05或P<0.01);皂苷组这5种基因水平均较空白组无明显差别;除PI3K外的4种基因,甲苷组较空白组有明显差别(P<0.05或P<0.01);而皂苷组与甲苷组水平也有统计学意义(P<0.05或P<0.01)。模型组与空白组、皂苷组相比,Fox O,PTEN水平明显升高(P<0.05或P<0.01),较甲苷组无明显差异;甲苷组这2种基因水平均较空白组升高显著(P<0.05);而皂苷组的Fox O高于空白组(P<0.05),皂苷组的PTEN与空白组无明显差异;皂苷组Fox O,PTEN水平与甲苷组无统计学意义。因此,该研究结果显示黄芪皂苷能提高Akt,PI3K,BCL-xl,bad,mTOR水平(P<0.01),降低Fox O,PTEN水平(P<0.05)。黄芪皂苷可有效改善环磷酰胺所造成的贫血,其机制可能与影响PI3K/Akt/mTOR信号通路相关基因有关。
To investigate the effect of astragaloside on mRNA expression of PI3K / Akt / mTOR related genes in chemotherapy-induced anemia mice. 48 BALB / c mice aged 6-7 weeks were selected and randomly divided into blank group, model group, saponin group and methyl glycoside group, with 12 in each group. The model of chemotherapy-induced anemia was established by cyclophosphamide. After intragastric administration for 14 days, blood was taken from the eye and the mRNA levels of Akt, PI3K, BCL-xl, bad, FoxO, mTOR and PTEN in the spleens were detected by QPCR. The results showed that the level of hemoglobin and hemoglobin in the model group was significantly lower than that in the blank group, the saponin group and the methylglycoside group (P <0.05). The levels of Akt, PI3K, BCL-xl, bad and mTOR in model group were significantly lower than those in blank group, saponin group and methylglycoside group (P <0.05 or P <0.01) There was no significant difference between the four genes except PI3K, methylglycosidase group and blank group (P <0.05 or P <0.01), while the levels of saponin and methyl glycosides also had statistical significance (P <0.05 or P < 0.01). The levels of FoxO and PTEN in model group were significantly higher than those in blank group and saponin group (P <0.05 or P <0.01) (P <0.05), while FoxO in saponin group was higher than that in blank group (P <0.05). There was no significant difference in PTEN between saponin group and blank group. FoxO and PTEN levels in saponin group were not significantly different from those in methylglycoside group . Therefore, the results of this study showed that astragaloside could increase the levels of Akt, PI3K, BCL-xl, bad and mTOR (P <0.01) and FoxO, PTEN (P <0.05). Astragaloside can effectively improve the anemia caused by cyclophosphamide, the mechanism may be related to the PI3K / Akt / mTOR signaling pathway related genes.