A simple, fast and reliable method was developed for the analysis ofjinggangmycin A (validamycin A) in commercial formulations. The running buffer used was acetate buffer (100mmol/L, pH 4.7) with 15 kV as the applied voltage. The detection was achieved by using direct UV mode at 200 nm and the detection limit was 0.2 μg/mL. Linearity in the concentration range of 5-500 μg/mL was excellent (R2>0.999). The run-to-run repeatability (n = 3), as expressed by the relative standard deviation (RSD) for migration times and peak areas were less than 0.5% and 3.0% respectively. The mean recovery ranged from 97.2% to 101.4%.