论文部分内容阅读
目的探讨尿路上皮癌乳腺癌易感基因1(BRCA1)mRNA水平与蛋白表达的相关性。方法收集50例尿路上皮癌患者标本组织进行石蜡切片3~5μm,采用免疫组织化学法检测BRCA1基因蛋白的表达。通过提取石蜡切片组织RNA进行反转录,采用实时定量PCR法检测BRCA1基因mRNA的表达。结果 BRCA1 mRNA低表达率64%,中表达率36%;BRCAl免疫组织化学染色阳性率为88%,阴性率为12%。采用SSPS 17.0统计学软件,经Wilcoxon两样本检验,BRCAl mRNA低表达组与中表达组的免疫组化染色程度差异有统计学意义(P<0.000 1);Spearman等级相关分析显示两者呈正相关性(rs=0.652 9)。结论实时荧光定量PCR检测mRNA表达与免疫组化检测蛋白表达皆为评估尿路上皮癌的可选方法。
Objective To investigate the correlation between the expression of BRCA1 mRNA and protein in urothelial carcinoma. Methods Fifty specimens of urothelial carcinoma were collected and paraffin sections were obtained. The expression of BRCA1 protein was detected by immunohistochemistry. Reverse transcription was performed by extracting RNA from paraffin sections, and the expression of BRCA1 mRNA was detected by real-time quantitative PCR. Results The low expression rate of BRCA1 mRNA was 64%, while the positive expression rate was 36%. The positive rate of BRCA1 immunohistochemical staining was 88% and the negative rate was 12%. Using SSPS 17.0 statistical software, Wilcoxon two-sample test showed that there was a significant difference in the immunohistochemical staining of BRCA1 mRNA between the low expression group and the middle expression group (P <0.0001); Spearman rank correlation analysis showed a positive correlation between the two (rs = 0.652 9). Conclusion Real-time fluorescence quantitative PCR detection of mRNA expression and immunohistochemical detection of protein expression are an alternative method for the evaluation of urothelial carcinoma.