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提要目的：研究SHEE生物学特性，包括增殖，分化和凋亡。方法：SHEE细胞系，199培基培养细胞，用光镜，电镜和荧光显微镜，免疫组化（Ki67，角蛋白），TdT标记，流式细胞仪和染色体分析。结果：细胞培养呈单层，锚定依赖和接触抑制生长。增殖指数PI34％，分裂指数MI2．74％（1
Abstract Objective: To study the biological characteristics of SHEE, including proliferation, differentiation and apoptosis. METHODS: SHEE cell line, 199 cells were cultured with light microscopy, electron microscopy and fluorescence microscopy, immunohistochemistry (Ki67, keratin), TdT labeling, flow cytometry and chromosome analysis. RESULTS: The cell culture was monolayer, anchored and contact inhibited growth. Proliferation index PI34%, split index MI2.74% (1