综述了水稻遗传转化体系研究 ,包括外植体的选择、报告基因和选择标记基因的选用、选用的启动子和基因转移方法。重点介绍了水稻转化系统应用进展 ,包括基因瞬时表达研究、基因表达调控研究、分析基因功能和构建基因标签、导入有益的农艺性状基因 4个方面 The research on rice genetic transformation system was reviewed, including the selection of explants, the selection of reporter and selectable marker genes, the selection of promoters and gene transfer methods. The application progress of rice transformation system is highlighted, including the study of gene transient expression, gene expression regulation, gene function analysis and gene tag construction, and introduction of useful agronomic trait genes