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目的:制备负载碱性成纤维细胞生长因子(bFGF)的壳聚糖微球,并测其体外释药特性。方法:采用乳化交联法制备壳聚糖空白微球,再进行bFGF吸附,制成负载bFGF的壳聚糖微球,并对微球进行扫描电镜观察。ELISA法测定其载药量与包封率,以及bFGF的体外释放情况。并测定壳聚糖空白微球的吸水膨胀率。结果:负载bFGF的壳聚糖微球直径约4μm,载药量为1.06μg/mg,包封率为84.8%。体外释放实验初期释放bFGF速度较快,随后逐渐趋于平缓。壳聚糖空白微球的吸水膨胀率为650%。结论:采用乳化交联法制备包裹bFGF的壳聚糖缓释微球,简单易行,成球性好,得率高,具有良好的缓释效果。
OBJECTIVE: To prepare chitosan microspheres loaded with basic fibroblast growth factor (bFGF) and to determine its in vitro release characteristics. Methods: Chitosan hollow microspheres were prepared by emulsion cross-linking method, and then bFGF was adsorbed to make chitosan microspheres loaded with bFGF. The microspheres were observed by scanning electron microscopy. ELISA method to determine the drug loading and encapsulation efficiency, and bFGF in vitro release. The swelling coefficient of chitosan blank microspheres was measured. Results: The chitosan microspheres loaded with bFGF had a diameter of about 4 μm, the drug loading was 1.06 μg / mg and the entrapment efficiency was 84.8%. In vitro release of bFGF early release rate faster, then gradually tends to be gentle. Chitosan hollow microspheres water swelling rate of 650%. CONCLUSION: Chitosan sustained-release microspheres coated with bFGF prepared by emulsification and cross-linking method are simple and easy to be formed, good in sphericity and high in yield, and have good sustained-release effect.