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目的 探讨用生物学方法治疗病毒感染性疾病及肿瘤的新途径。方法 以有重要补体活化调节功能的膜补体调节蛋白CD5 5为靶点 ,以 β GaL为模拟病毒或肿瘤抗原 ,制备“IgG”型抗CD5 5×抗 β Gal基因工程双特异性抗体 ,并对该重组抗体真核表达后的结合活性进行初步的验证。结果 克隆的CD5 5抗体可变区片段为新的小鼠抗体可变区片段 ,经HEK 2 93细胞表达后的重组抗体显示了良好的CD5 5及Fc结合活性。结论 本研究为病毒性疾病或肿瘤的免疫学治疗提供了新的途径及实验依据
Objective To explore new ways to treat viral infectious diseases and tumors with biological methods. Methods Anti-CD5 5 × anti-β Gal genetically engineered bispecific antibody was prepared by targeting β GaL as a mimic virus or tumor antigen with membrane complement regulatory protein CD5 5 as an important regulator of complement activation. The recombinant antibody eukaryotic expression of the binding activity of preliminary validation. Results The cloned variable region of CD5 5 antibody was a new variable fragment of mouse antibody. The recombinant antibody expressed by HEK2 93 cells showed good CD5 5 and Fc binding activity. Conclusion This study provides a new way and experimental basis for the immunological treatment of viral diseases or tumors