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目的:探讨缬草水提物对小鼠外周血单核细胞的白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)mRNA表达的影响。方法:小鼠经缬草水提物灌胃给药1周后,采用逆转录PCR(RT-PCR)方法检测外周血单核细胞中的IL-1β和TNF-αmRNA的表达情况。结果:缬草小、中、大剂量组给药后,小鼠腹腔巨噬细胞IL-1βmRNA表达均增高,与空白对照组比较有差异(P<0.05)。缬草中、大剂量组给药后,TNF-αmRNA表达增高,与空白对照组比较有差异(P<0.05)。结论:缬草能够增加小鼠外周血单核细胞表达IL-1β和TNF-α的数量,从而起到催眠的作用。
Objective: To investigate the effects of Valerian water extract on the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) mRNA in peripheral blood mononuclear cells of mice. Methods: The mice were treated with water extract of valerian root for one week, and the expression of IL-1β and TNF-αmRNA in peripheral blood mononuclear cells were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results: The expression of IL-1β mRNA in peritoneal macrophages increased after administration of small, medium and large doses of valerian, which was significantly different from that of the blank control group (P <0.05). In valerian medium and high dose groups, the expression of TNF-αmRNA increased, compared with the blank control group (P <0.05). Conclusion: Valerian can increase the expression of IL-1β and TNF-α in peripheral blood mononuclear cells of mice and thus play a hypnotic role.