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A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles(PNA/Ag NPs) has been initially proposed for specific recognition of m RNA.Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc m RNA detection can be achieved. Moreover, the PNA/Ag NPs platform can undergo color change in response to target c-Myc m RNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.
A label-free colorimetric protocol based on peptide nucleic acid / silver nanoparticles (PNA / Ag NPs) has been initially proposed for specific recognition of m RNA. Making use of the controlled silver nanoparticles aggregation / dispersion by PNA / PNA-RNA complex, proto -oncogene c-Myc m RNA detection can be achieved. Moreover, the PNA / Ag NPs platform can under color change in response to target c-Myc m RNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.