论文部分内容阅读
为获得罗非鱼腹腔巨噬细胞体外分离培养条件和培养形态特征,试验在不同饲养温度和时间点注射角鲨烯的奥尼罗非鱼进行腹腔巨噬细胞分离;通过培养基血清筛选和瑞氏染色对分离的巨噬细胞进行了体外培养及其形态观察。结果表明,19～25℃饲养温度有利于腹腔巨噬细胞分离,大于28℃对分离效果有明显影响;注射角鲨烯后48～72 h是分离腹腔巨噬细胞最佳时期;体外培养显示自体血清有利于罗非鱼腹腔巨噬细胞的存活与生长,胎牛血清不适于罗非鱼腹腔巨噬细胞培养。同时,罗非鱼腹腔巨噬细胞体外培养具有哺乳动物巨噬细胞相似特征,形态不规则、核浆比值低、贴壁生长、可形成多核巨大细胞。为下一步研究罗非鱼腹腔巨噬细胞免疫学功能奠定了基础。
In order to obtain the culture conditions and morphological characteristics of tilapia peritoneal macrophages in vitro, the peritoneal macrophages were isolated from Orenio tilapia injected with squalene at different feeding temperatures and time points. The isolated macrophages were cultured in vitro and their morphology was observed. The results showed that the feeding temperature of 19-25 ℃ was beneficial to the separation of peritoneal macrophages, and the effect of separation was obvious when the temperature was higher than 28 ℃. 48-72 h after injection of squalene was the best period for separating peritoneal macrophages. Serum is conducive to the survival and growth of tilapia peritoneal macrophages, fetal bovine serum is not suitable for tilapia peritoneal macrophage culture. At the same time, tilapia peritoneal macrophages cultured in vitro with similar characteristics of mammalian macrophages, irregular shape, nuclear ratio of nuclear low, adherent growth, the formation of multi-nuclear giant cells. Which laid the foundation for further study on the immunological function of tilapia peritoneal macrophages.