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采用~３Ｈ－ＴｄＲ、~３Ｈ－脯氨酸、ＤＮＡ定量检测及ＭＴＴ比色等方法，研究猪肺动脉缺氧性内皮细胞条件培养液（ＨＥＣＣＭ）对猪肺动脉平滑肌细胞（ＰＡＳＭＣ）增生及胶原蛋白合成的影响，并探讨丹参对此过程的阻抑作用。结果表明：ＨＥＣＣＭ能引起ＰＡＳＭＣ的线粒体酶活性升高，核内ＤＮＡ含量增加及~３Ｈ－ＴｄＲ、~３Ｈ－脯氨酸掺入量增多。ＨＥＣＣＭ组ＰＡＳＭＣ的~３Ｈ－脯氨酸掺入量是常氧性内皮细胞条件培养液（ＮＥＣＣＭ）组的１．８３倍。中药丹参注射液能阻抑ＨＥＣＣＭ引起的ＰＡＳＭＣ增生和降低ＨＥＣＣＭ组和ＮＥＣＣＭ组ＰＡＳＭＣ的~３Ｈ－脯氨酸掺入量。但不影响ＮＥＣＣＭ组ＰＡＳＭＣ核的ＤＮＡ含量及~３Ｈ－ＴｄＲ掺入量。提示缺氧可能会刺激肺动脉内皮细胞（ＰＡＥＣ）合成、分泌某些能促进ＰＡＳＭＣ增生及胶原蛋白合成的细胞因子，丹参有抑制此过程的效应。
The proliferation of porcine pulmonary arterial smooth muscle cells (PASMC) and collagen synthesis were studied using porcine pulmonary artery hypoxic endothelial cell conditioned media (HECCM) using ~3H-TdR, ~3H-proline, quantitative DNA detection, and MTT colorimetry. The influence of Salvia miltiorrhiza on the inhibition of this process. The results showed that HECCM could increase the mitochondrial enzyme activity of PASMC, increase the nuclear DNA content and increase the incorporation of ~3H-TdR and ~3H-proline. The ~3H-proline incorporation of PASMC in the HECCM group was 1.83 times that of the normoxic endothelial cell conditioned medium (NECCM) group. Chinese medicine Salvia miltiorrhiza injection can inhibit the proliferation of PASMC induced by HECCM and reduce the 3H-proline incorporation of PASMC in the HECCM and NECCM groups. However, it did not affect the DNA content and ~3H-TdR incorporation of PASMC nucleus in NECCM group. It is suggested that hypoxia may stimulate the synthesis of pulmonary artery endothelial cells (PAEC) and secrete certain cytokines that can promote the proliferation of PASMC and collagen synthesis. Salvia miltiorrhiza has the effect of inhibiting this process.