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目的观察原花青素(proanthocyanidin,PC)对氧化应激(oxidative stress)致大鼠心肌缺血再灌注损伤的保护作用。方法采用结扎大鼠冠状动脉左前降支(LAD)30min,复灌3h的方法,建立大鼠心肌缺血再灌注损伤模型,对照组、剂量组分别给予生理盐水、PC,阳性对照组给予银杏叶片(48mg/kg),观察PC对大鼠心肌梗死面积、心肌细胞凋亡及心肌酶学的影响。结果与假手术组比较,模型对照组大鼠的心肌梗死面积明显增大、心肌细胞凋亡指数与心肌酶学各指标均发生显著变化,差异均有统计学意义(P<0.01);与模型对照组比较,阳性对照组、PC剂量组降低了心肌缺血再灌注损伤模型大鼠的心梗面积、心肌细胞凋亡、减少心肌细胞磷酸肌酸激酶(CK)、乳酸脱氢酶(LDH)释放、心肌组织黄嘌呤氧化酶(XO)活性及血清丙二醛(MDA)含量,提高了血清一氧化氮(NO)水平、血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及谷胱甘肽(GSH)含量,差异有统计学意义(P<0.05或P<0.01),并存在剂量反应关系。结论 PC对心肌缺血再灌注损伤模型大鼠具有抗氧化应激作用,能够减少心肌细胞凋亡,从而减少心肌梗死面积,并存在剂量反应关系。
Objective To observe the protective effect of proanthocyanidin (PC) on myocardial ischemia-reperfusion injury induced by oxidative stress in rats. Methods The rat model of myocardial ischemia-reperfusion injury was established by ligating the left anterior descending coronary artery (LAD) for 30 minutes and reperfusion for 3 hours in rats. The rats in the control group and the dose group were given saline, PC, and the positive control group was given Ginkgo biloba (48mg / kg) to observe the effect of PC on myocardial infarct size, cardiomyocyte apoptosis and myocardial enzyme in rats. Results Compared with the sham-operation group, the area of myocardial infarction in the model control group increased significantly, the index of myocardial apoptosis index and the index of myocardial enzyme changed significantly (P <0.01) Compared with the control group, the positive control group and the PC dose group reduced the myocardial infarction area, myocardial cell apoptosis, decreased the myocardial cell phosphorylated creatine kinase (CK) and lactate dehydrogenase (LDH) in the myocardial ischemia reperfusion injury model rats, Release, the activity of xanthine oxidase (XO) and the content of serum malondialdehyde (MDA) in myocardium, and the levels of serum nitric oxide (NO), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and glutathione (GSH) content, the difference was statistically significant (P <0.05 or P <0.01), and the existence of dose-response relationship. Conclusion PC has anti-oxidative stress effects on myocardial ischemia-reperfusion injury in rats, which can reduce the myocardial apoptosis and reduce the myocardial infarction area, and there is dose-response relationship.