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目的 研究血小板激活因子 (PAF)在急性脑缺血过程中的作用。方法 用高效薄层层析法对大鼠全脑缺血后再灌注 1、3、6、72小时血液、海马、大脑顶叶皮质的 PAF进行了观察。结果 再灌注 1小时后海马中 PAF含量较对照组明显升高 (P<0 .0 1) ,而 3小时时则下降至正常水平 (P>0 .0 5 )。血液中 PAF的升高出现较晚 ,再灌注后 3小时时血中 PAF明显升高 ,6小时时最明显 ,72小时时与对照组比较仍有显著性差异(P<0 .0 5 )。结论 脑缺血后海马 PAF的变化快而短暂 ,早期血液中 PAF可能主要来自脑组织 ,与血脑屏障破坏有关。提示 PAF参与了脑缺血早期的病理生理过程
Objective To study the role of platelet activating factor (PAF) in acute cerebral ischemia. Methods The PAF of blood, hippocampus and parietal cortex in rats after 1, 3, 6, 72 hours of reperfusion after global cerebral ischemia were observed by high performance thin layer chromatography. Results The level of PAF in hippocampus was significantly increased (P <0.01) at 1 hour after reperfusion and decreased to normal level at 3 hours (P> 0.05). Blood PAF increased later, at 3 hours after reperfusion PAF was significantly elevated in blood 6 hours, the most obvious, 72 hours compared with the control group there are still significant differences (P <0. Conclusion The change of PAF in hippocampus after cerebral ischemia is fast and transient. PAF in early blood may mainly come from brain tissue, which is related to the destruction of blood-brain barrier. Suggesting that PAF is involved in the pathophysiology of early cerebral ischemia