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根据鉴别寄主反应、寄主范围测定、介体传播特性、粒体形态以及血清学诸项试验,将蚕豆病毒分离物B_5鉴定为黄瓜花叶病毒(Cucumber Mosaic virus,CMV)。B_5液接种能侵染7科20余种植物。在蚕豆的接种叶上形成红褐色坏死斑,有或无系统侵染;局部侵染苋色藜和昆诺藜;系统侵染心叶烟和番茄分别引起花叶和蕨叶症。B_5可经桃蚜、棉蚜和甘篮蚜的非持久方式传播。B_3在电镜下粒体形态为球状,直径28～30nm。B_5病株的粗制液在免疫双扩散试验中能和黄瓜花叶病毒的抗血清起反应,且在免疫电镜试验中,黄瓜花叶病毒抗血清处理的铜网能捕获大量B_5粒体。
The isolate B_5 of Vicia faba was identified as Cucumber Mosaic Virus (CMV) according to the tests of host reaction, host range, mediator spread, morphology and serology. B_5 liquid inoculation can infect more than 20 species of seven families of plants. Red brown necrotic spots were formed on the inoculated leaves of Vicia faba, with or without systemic infection; local inoculation of Amaranthus veratris and Quinoa; systemic infection of leaf-leaf and tomato caused mosaic and fern leaf diseases, respectively. B_5 can be transmitted by peach aphid, cotton aphid and aphid in the non-durable way. B_3 under electron microscopy spherical shape, diameter 28 ~ 30nm. The crude solution of B_5 strain could react with cucumber mosaic virus antiserum in the double immunodiffusion test. In the immunoelectron microscopy, copper cucumber mosaic virus antiserum could capture a large amount of B_5 particles.