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目的 :探讨乙型肝炎患者外周血淋巴细胞 (peripheralbloodlymphocytes,PBLs)凋亡在其慢性化、重型化过程中的作用。方法 :分离 2 0例正常人、2 4例慢性乙型肝炎患者及 2 4例慢性重型乙型肝炎患者之PBLs ,体外单独或与植物血凝素 (PHA)共同培养 4 8h ,用荧光素吖啶橙和溴乙啶染色PBLs,观察其凋亡细胞百分率。结果 :慢性乙型肝炎患者与正常对照组静息的淋巴细胞凋亡率都比较低 ,两组比较差异无显著性 (P >0 .0 5 ) ;但与正常对照组相比 ,活化诱导的淋巴细胞凋亡在慢性乙型肝炎患者明显增高 ,而在慢性重型肝炎患者则降低 ,差异有显著性 (P <0 .0 1)。结论 :慢性乙型肝炎患者外周血淋巴细胞存在凋亡紊乱现象 ,这可能是导致乙型肝炎慢性化、重型化的重要因素之一。
Objective: To investigate the role of apoptosis of peripheral blood lymphocytes (PBLs) in chronic hepatitis B patients and patients with severe hepatitis B virus infection. Methods: PBLs from 20 normal individuals, 24 chronic hepatitis B patients and 24 severe chronic hepatitis B patients were isolated and cultured in vitro for 48 h with phytohemagglutinin (PHA). Fluorescein acridine Pyridine orange and ethidium bromide staining PBLs, observe the percentage of apoptotic cells. Results: The rate of apoptotic lymphocytes resting in patients with chronic hepatitis B was significantly lower than that of normal controls (P> 0.05), but compared with the normal control group, the activation induced Lymphocyte apoptosis was significantly increased in patients with chronic hepatitis B, while in patients with chronic severe hepatitis, the difference was significant (P <0.01). Conclusion: Apoptosis of peripheral blood lymphocytes in patients with chronic hepatitis B is abnormal, which may be one of the important factors leading to chronic and severe hepatitis B infection.