目的　从离子通道水平 ,探讨前列腺素E1(PGE1)对ATP敏感K+ (KATP)通道的作用。方法　膜片钳制技术全细胞记录模式。结果　PGE1可诱导KATP通道开放并呈浓度依赖关系。保持电位 - 40mV ,指令电位 +2 0mV ,持续时间 1s条件下 ,10 μmol·L-1PGE1使外向K+ 电流由给药前的 (2 2 7± 0 34 )nA增加到 (5 46± 0 34 )nA(n =6 ,P <0 0 1) ,增加了 (3 18± 0 2 3)nA。并且增加的钾电流可被KATP通道特异阻断剂Glibenclamide(10 μmol·L-1)抑制 ,抑制率是 6 3%± 7% (n =5 ,P <0 0 1)。结论　PGE1可开放豚鼠心室肌细胞KATP通道 Objective To investigate the effect of prostaglandin E1 (PGE1) on ATP-sensitive K + channel in ion channels. Methods Patch-clamp technique Whole-cell recording mode. Results PGE1 induced KATP channel opening in a concentration-dependent manner. The potential of 10 μmol·L-1 PGE1 was increased from (2 2 7 ± 0 34) nA to (5 46 ± 0 34) nM before administration at the holding potential of -40 mV and the command potential of +20 mV for 1 s, nA (n = 6, P <0 0 1) increased by (3 18 ± 0 2 3) nA. And the increase of K (superscript +) current was inhibited by Glibenclamide (10 μmol·L-1), the inhibition rate was 63% ± 7% (n = 5, P <0.01). Conclusion PGE1 can open the KATP channel of guinea pig ventricular myocytes