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设计并合成了一对聚合酶链反应（ＰＣＲ）的通用引物，可以从小鼠杂交瘤的ｃＤＮＡ中扩增出免疫球蛋白（Ｉｇ）Ｋ链可变区（ＶＫ）基因。利用这一对引物和原位突变技术，在小鼠的一个ｇｅｎｏｍｉｃＶＫ基因的两端分别引入一个ＤＮＡ限制性内切酶的酶切位点，构建成一个ＩｇＶＫ基因的通用型克隆载体。并将ＶＫ基因片段同人ｋ链基因区基因片段重组，构建成人－鼠嵌合ｋ链基因表达载体。
A universal primer pair for polymerase chain reaction (PCR) was designed and synthesized to amplify immunoglobulin (Ig) K chain variable region (VK) gene from mouse hybridoma cDNA. Using this pair of primers and in situ mutagenesis, a DNA restriction endonuclease site was introduced into each genomicVK gene of mice to construct a universal cloning vector for IgVK gene. The VK gene fragment was recombined with the human kappa chain gene fragment to construct a human-mouse chimeric k-chain gene expression vector.