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目的探讨姜黄素对前脂肪细胞葡萄糖摄取、分化的影响及其机制。方法用高浓度葡萄糖、胰岛素联合诱导培养的方法,建立脂肪细胞胰岛素抵抗模型并检测姜黄素、干预的脂肪细胞对~3H-葡萄糖的摄取,对姜黄素干预分化的细胞进行油红O染色并通过比色定量分析脂肪细胞分化程度。实时定量荧光PCR检测脂肪细胞分化相关基因过氧化物体增殖剂活化受体γ(PPARγ)和CAAT/增强子结合蛋白A(C/EBPα)mRNA的表达。结果姜黄素组葡萄糖摄取率为正常组的134.51%;姜黄明显促进脂肪细胞分化及PPARγ和C/EBPαmRNA其表达,与对照组比较,差异有统计学差异(P<0.05)。结论姜黄素能够促进脂肪细胞葡萄糖摄取及细胞分化,PPARγ和C/EBPαmRNA表达。
Objective To investigate the effect of curcumin on glucose uptake and differentiation in preadipocytes and its mechanism. Methods The model of adipocyte insulin resistance was established by the combination of high concentration of glucose and insulin. The uptake of ~ 3H-glucose was detected by curcumin and intervention of adipocytes. Oil red O staining was performed on the cells differentiated by curcumin and passed Colorimetric quantitative analysis of adipocyte differentiation. Real - time quantitative PCR was used to detect the mRNA expression of peroxisome proliferator - activated receptor γ (PPARγ) and CAAT / enhancer binding protein A (C / EBPα) in adipocyte differentiation related genes. Results The glucose uptake rate in curcumin group was 134.51% of that in normal group. Turmeric markedly promoted the differentiation of adipocytes and the expression of PPARγ and C / EBPαmRNA. Compared with the control group, the difference was statistically significant (P <0.05). Conclusion Curcumin can promote glucose uptake and cell differentiation, PPARγ and C / EBPα mRNA expression in adipocytes.