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本文系根据急性胰腺炎的发病之一,即胆汁反流学说而设计一种方法,在实验动物中造成急性出血性胰腺炎模型,以供研究急性胰腺炎的发病机制。作者等在大鼠的胆胰管内注射结合胆盐(牛磺胆酸钠),可无例外地引起致命的急性出血性胰腺炎。材料与方法:实验用96只雌、雄性Wistar鼠,体重在180～200g,分为6组。可随意饮水、进食。打开腹腔,在胰管系统内注射0.2ml生理盐水或0.2ml牛磺胆酸钠(用三种不同浓度)。用手推压力,在60秒内注完。手术后在背部皮下注射4ml生理盐水,以补充手术中液体的丢失。第1组:7只大鼠,注入生理盐水0.2ml。分别于术后1分钟、15分钟及1、3、6、12、24小时,用乙醚过量的方法处死1只。第2组:21只大鼠,注入5％牛磺胆酸钠溶液0.2ml。如第1组的间隔时间,每次处死3只。第3组:15只大鼠、管内注入生理盐水0.2ml。第4组:17只大鼠,注入3％牛磺胆酸钠溶
This article is based on the pathogenesis of acute pancreatitis, one of the pathogenesis of acute pancreatitis, that is, to design a method for acute hemorrhagic pancreatitis in experimental animals for the study of the pathogenesis of acute pancreatitis. The authors injected rat biliary and pancreatic ducts with bile salts (sodium taurocholate), which can cause fatal acute hemorrhagic pancreatitis without exception. MATERIALS AND METHODS: Ninety-six female and male Wistar rats, weighing 180-200 g, were divided into six groups. Feel free to drink water and eat. Open the abdominal cavity and inject 0.2 ml saline or 0.2 ml sodium taurocholate (in three different concentrations) in the pancreatic duct. Hand pressure, note within 60 seconds. After surgery, subcutaneous injection of 4ml saline in the back to supplement the loss of liquid during surgery. Group 1: 7 rats, saline 0.2ml. One patient was sacrificed at 1 minute, 15 minutes and 1, 3, 6, 12, and 24 hours after operation, respectively. Group 2: 21 rats were injected with 0.2% sodium taurocholate solution 5%. If the first group of intervals, each killed three. Group 3: 15 rats, the tube into the physiological saline 0.2ml. Group 4: 17 rats injected with 3% sodium taurocholate dissolved