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本实验对番茄组织培养过程中分化产生的愈伤组织、根、芽、茎、叶等进行了过氧化物酶同工酶分析，并以生长１５－２０天的正常番茄苗的根、茎、叶材料作为对照。结果表明，组培植物的过氧化物酶同工酶酶谱与对照有较明显变化，组培材料酶谱带染色较深，其中根中Ａ—３—α，Ｂ—１—α带，愈伤组织的Ａ—３—α带显示最深，组培叶显示了１７条带，而对照叶仅显示了５条带；茎显示１７条带，而对照显示６条带，尤其是组培材料均显示出Ｂ—３带，而对照材料没有此条带．上述结果对探讨组织培养过程中分化的生理基础具有一定意义．
In this experiment, peroxidase isoenzyme analysis was conducted on the callus, root, bud, stem, leaf and so on during differentiation of tomato tissue culture. The root, stem, Leaf material as a control. The results showed that the peroxidase isozyme zymogram of tissue culture plants had obvious changes compared with the control. The zymogram bands of tissue culture materials were deeply stained. Among them, A-3-α and B-1-α bands in root The A-3-alpha band in the wounded tissue showed the deepest, tissue culture leaf showed 17 bands, whereas the control leaf showed only 5 bands; stems showed 17 bands whereas the control showed 6 bands, especially tissue culture material The B-3 band is shown, whereas the control material does not have this band. The above results have some significance to explore the physiological basis of differentiation during tissue culture.