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目的研究人胃癌组织淋巴管的超微结构特点,探讨胃癌淋巴转移机制。方法取人胃癌中心区、周边区及正常区组织块,常规固定,树脂包埋,半薄切片,光镜下定位,检出淋巴管经超薄切片,透射电镜观察。结果人胃癌中心区未见淋巴管;胃癌周边区较正常区淋巴管增多(t=3.270,P=0.002),管腔较小(t=-5.315,P=0.000),开放连接增多(χ2=12.895,P=0.000),淋巴管破坏增多(χ2=15.674,P=0.000);淋巴转移组胃癌周边区比无淋巴转移组胃癌周边区淋巴管多(t=2.523,P=0.021),被破坏的淋巴管多(χ2=5.281,P=0.022),开放连接/非开放连接数差异无统计学意义(χ2=0.217,P=0.641)。结论人胃癌周边区存在淋巴管新生;胃癌细胞可能是通过胃癌周边区被破坏的淋巴管进行转移的。
Objective To study the ultrastructural characteristics of lymphatic vessels in human gastric cancer and to explore the mechanism of lymphatic metastasis in gastric cancer. Methods The tissue sections of the central, peripheral and normal human gastric cancer tissues were obtained and fixed by routine fixation, resin-embedded, semi-thin section and light microscopy. The lymphatic vessels were detected by ultrathin sections and transmission electron microscopy. Results There were no lymphatic vessels in the central area of human gastric cancer. The lymphatic vessels were increased in the peripheral area of gastric cancer as compared with the normal area (t = 3.270, P = 0.002), and the lumen was smaller (t = -5.315, P = 0.000) (Χ2 = 15.674, P = 0.000). The lymphatic metastasis in lymphatic metastasis group was more than that in the lymphatic metastasis group (t = 2.523, P = 0.021) (Χ2 = 5.281, P = 0.022). There was no significant difference between open and non-open connections (χ2 = 0.217, P = 0.641). Conclusion Lymphangiogenesis exists in the peripheral region of human gastric cancer. Gastric cancer cells may be metastasized through lymphatic vessels damaged in the peripheral region of gastric cancer.