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目的探讨谷氨酰胺(glutamine,Gln)对新生鼠坏死性小肠结肠炎(necrotizing enterocoli-tis,NEC)肠黏膜修复的影响。方法新生1日龄Wistar大鼠30只随机分为三组,A组为正常对照组;B组为NEC模型组;C组为NEC模型后灌胃给Gln组(2·0g/kg·d)。建立NEC模型,连续3天给予新生鼠100%二氧化碳5min,然后再给予100%氧气5min,放回母鼠身边喂养,第4天断头处死新生鼠,取肠道组织待检。分别取近回盲段2~3cm肠道组织固定、包埋、切片。HE染色光镜下作病理学检查,应用免疫组化技术检测肠黏膜增殖细胞核抗原(PCNA)表达情况,原位末端标记(TUNEL)法检测肠黏膜细胞凋亡的变化。结果B组HE染色切片见肠壁有不同程度的损伤,病理评分的中位积分为3分;C组有的肠黏膜正常,有的轻度肠上皮细胞脱落,有的绒毛轻度坏死,病理评分的中位积分为1分。B组PCNA数量均低于A组及C组(P<0·01)。B组的肠黏膜细胞凋亡的数量高于A组及C组(P<0·01)。结论NEC时,新生鼠肠黏膜受损,增殖减慢,细胞凋亡的数量增加;补充Gln可促进NEC新生鼠肠黏膜隐窝细胞增殖,减少肠黏膜细胞凋亡数量,使肠黏膜修复加快。
Objective To investigate the effect of glutamine (Gln) on intestinal mucosal repair in neonatal necrotizing enterocolisis (NEC). Methods Thirty newborn Wistar rats were randomly divided into three groups, group A was normal control group, group B was NEC model group, group C was NEC model, and fed with Gln group (2.0 g / kg · d) . The model of NEC was established. Neonatal rats were given 100% carbon dioxide for 5 min for 3 consecutive days and then given 100% oxygen for 5 min. The rats were fed back to the mother rats. The newborn rats were sacrificed on the 4th day and intestine tissues were taken for examination. Were taken near the ileocecal 2 ~ 3cm intestinal tissue fixed, embedded, sliced. Pathological examination was performed under HE staining. The expression of proliferating cell nuclear antigen (PCNA) in intestinal mucosa was detected by immunohistochemistry. The apoptosis of intestinal mucosa was detected by TUNEL. Results The histopathological changes in group B were observed by HE staining. The median score of pathological score was 3; the intestinal mucosa in group C was normal, some mild intestinal epithelial cells were shed, some were slightly necrotic and pathological The median score for the score is 1 point. The number of PCNA in group B was lower than that in group A and C (P <0.01). The number of intestinal mucosal apoptosis in group B was higher than that in group A and C (P <0.01). Conclusions The intestinal mucosa of neonatal rats were impaired and the proliferation slowed down and the number of apoptotic cells increased when NEC was added. Supplementation of Gin could promote the proliferation of intestinal mucosal crypt cells in neonatal rats with NEC and reduce the number of intestinal mucosa apoptosis, which accelerated the repair of intestinal mucosa.