论文部分内容阅读
目的:建立脑塞通丸的含量测定方法。方法:采用高效液相色谱法。色谱柱:Agilent Technologies ZORBAX Extend- C_(18)(250 mm×4.6 mm,5μm),流动相:乙腈-0.05%磷酸溶液梯度洗脱,流速:1.0 ml·min~(-1),检测波长:203 nm,柱温:35℃。结果:人参皂苷Rg_1在8.516～425.8μg·ml~(-1)范围内线性关系良好(r=0.9999),平均加样回收率为97.6%,RSD=1.7%(n =6);人参皂苷Re在20.06～1003μg·ml~(-1)范围内线性关系良好(r=0.9997),平均加样回收率为97.6%,RSD=2.0%(n= 6);人参皂苷Rb_1在19.456～972.8μg·ml~(-1)范围内线性关系良好(r=0.9997),平均加样回收率为96.8%,RSD=1.3%(n= 6)。结论:该方法简便易行,结果准确可靠,可适用于脑塞通丸的质量控制。
Objective: To establish a method for determining the content of Naositong pill. Method: High performance liquid chromatography. Column: Agilent Technologies ZORBAX Extend-C_(18) (250 mm×4.6 mm, 5 μm), mobile phase: acetonitrile-0.05% phosphoric acid gradient elution, flow rate: 1.0 ml·min-1, detection wavelength: 203 nm, column temperature: 35°C. Results: The ginsenoside Rg_1 had good linearity in the range of 8.516～425.8μg·ml -1 (r=0.9999). The average recovery was 97.6%, RSD was 1.7% (n = 6); Ginsenoside Re The linearity was good in the range of 20.06～1003μg·ml -1 (r=0.9997), the average recovery was 97.6%, RSD was 2.0% (n=6), and the ginsenoside Rb_1 was 19.456～972.8μg. The linear range of ml~(-1) was good (r=0.9997), the average recovery was 96.8%, RSD=1.3% (n=6). Conclusion: This method is simple, accurate and reliable, and can be applied to the quality control of Naosi Tongwan.