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目的 探讨人膀胱移行细胞癌与高危型人乳头瘤病毒感染的关系。方法 采用聚合酶链式反应 (PCR法 )检测 112例膀胱移行细胞癌组织 (包括 75例石蜡包埋组织和 3 7例手术切除组织 )和 7例正常膀胱粘膜组织的HPV 16/18感染率及HPV 16/18感染与膀胱移行细胞癌病理分级及临床分期的关系。同时检测了 2 4例膀胱癌病人尿液沉淀中HPV阳性率。结果 膀胱移行细胞癌的HPV 16/18总感染率为 62 .5 0 % ( 70 /112 ) ,与正常对照相比有显著性差异 (P <0 .0 5 )。 2 4例膀胱癌病人尿液沉淀中的HPV 18检出率为 41.67% ( 10 /2 4)。但膀胱病理分级、临床分期与HPV感染无关 (P >0 .0 5 )。结论 膀胱移行细胞癌的发生与高危型人乳头瘤病毒HPV感染可能相关 ,且很可能是膀胱移行细胞癌的早期事件之一。
Objective To investigate the relationship between human bladder transitional cell carcinoma and high-risk human papillomavirus infection. Methods The HPV 16/18 infection rate in 112 cases of bladder transitional cell carcinoma (including 75 cases of paraffin-embedded tissues and 37 cases of resected tissues) and 7 cases of normal bladder mucosa were detected by polymerase chain reaction (PCR) Relationship Between HPV 16/18 Infection and Pathological Grading and Clinical Staging of Bladder Transitional Cell. At the same time, the positive rate of HPV in urine sediment of 24 bladder cancer patients was detected. Results The total infection rate of HPV 16/18 in bladder transitional cell carcinoma was 62.5% (70/112), which was significantly different from that of normal control (P <0.05). The detection rate of HPV 18 in 24 cases of urinary bladder cancer was 41.67% (10/2 4). However, bladder pathological grading, clinical stage has nothing to do with HPV infection (P> 0.05). Conclusion The occurrence of transitional cell carcinoma of the bladder may be associated with HPV infection of high-risk human papillomavirus and may be one of the early events of bladder transitional cell carcinoma.