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目的：探讨标记蛋白质在肾移植急性细胞性排异（ＡＣＲ）时的作用及其临床诊断意义。方法：选择一组具有重要生物学功能的蛋白质，如ＰＣＮＡ、Ｂｃｌ－２、ＩＬ－２Ｒ（ＣＤ２５）、ＩＣＡＭ－１、ＨＬＡ－ＤＲ等作为标记蛋白，着重观察移植肾发生ＡＣＲ时，肾小管和间质浸润细胞中上述标记蛋白的变化，及其与间质浸润ＣＤ４或ＣＤ８细胞的关系，并与未发生排异反应者作对照。结果：无ＡＣＲ的肾组织中，ＰＣＮＡ和ＤＲ蛋白仅轻度增加，Ｂｃｌ－２、ＩＬ－２Ｒ和ＩＣＡＭ－１的增加不明显。当移植肾出现ＡＣＲ时，上述标记蛋白均明显增加，尤以ＰＣＮＡ和ＩＬ－２Ｒ增加最为显著，且与间质浸润的ＣＤ８细胞密切相关。结论：鉴于ＩＬ－２Ｒ具有的独特免疫学作用，以及变化的选择性和特异性，作者认为ＩＬ－２Ｒ对于ＡＣＲ的诊断及鉴别诊断具有一定的临床应用价值
Objective: To investigate the role of marker protein in acute renal transplantation rejection (ACR) and its clinical significance. Methods: A group of proteins with important biological functions such as PCNA, CD25, ICAM-1 and HLA-DR were selected as the marker proteins to observe the changes of renal tubular and Interstitial infiltrating cells in the above marker protein changes, and its relationship with interstitial infiltration of CD4 or CD8 cells, and with no rejection reaction as a control. RESULTS: PCNA and DR protein increased only mildly in ACR-deficient renal tissues, with no significant increase in Bcl-2, IL-2R and ICAM-1. When ACR was detected in renal allografts, the above-mentioned marker proteins were significantly increased, especially in PCNA and IL-2R, and were closely related to interstitial infiltrating CD8 cells. CONCLUSION: In view of the unique immunological effects of IL-2R and the selective and specific changes, IL-2R is of clinical value in the diagnosis and differential diagnosis of ACR