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研究了2003年夏季长白赤松和红松土壤微生物活性对高浓度CO2的响应规律.结果表明,长白赤松和红松土壤细菌数量受高浓度CO2影响显著(p < 0.05)减少;与对照箱(350 μmol · mol-1 CO2)和裸地(350 μmol · mol-1 CO2)相比,红松土壤淀粉酶和转化酶活性降低,而长白赤松土壤淀粉酶和转化酶活性却表现为增加;同时发现受700 μmol · mol-1 CO2处理的红松和长白赤松土壤微生物生物量碳均表现为显著降低.DGGE结果表明:受高浓度CO2的影响,长白赤松和红松土壤细菌群落结构发生了明显的变化.研究结果表明土壤微生物对高浓度CO2的响应规律与所研究的的树种有关.图2表2参29.“,”Responses of soil microbial activities to elevated CO2 in experiment sites of Pinus sylvestriformis and Pinus koraiensis seedlings were studied in summer in 2003. The results indicated the number of bacteria decreased significantly (p < 0.05) under elevated CO2 for Pinus sylvestriformis and Pinus koraiensis. Amylase and invertase activities in soil increased for Pinus sylvestriformis and decreased for Pinus koraiensis with CO2 enrichment compared with those at ambient (350 μmol·mol-1). The size of microbial biomass C also decreased significantly at 700 μmol·mol-1 CO2. Bacterial community structure had some evident changes under elevated CO2 by DGGE (Denaturing Gradient Gel Electrophoresis) analysis of bacterial 16S rDNA gene fragments amplified by PCR from DNA extracted directly from soil. The results suggested that responses of soil microorganisms to elevated CO2 would be related to plant species exposed to elevated CO2.