目的制备抗人CD28单克隆抗体,对其免疫学特性进行分析。方法利用多肽技术合成的CD28抗原免疫BALb/c小鼠,应用细胞杂交技术,获得抗CD28的单克隆抗体,并对其免疫学特性进行了分析。结果经过融合、亚克隆成功获得了9株抗人CD28的杂交瘤细胞株,均为IgG,4株为IgG2a,5株为IgG2b。利用竞争抑制实验分别对其中的5株进行了抗原位点测定,5株分别针对三个不同的抗原位点。结论此单抗细胞株的建立,对于深入研究CD28单抗对T淋巴细胞的作用机理有重要意义。 Objective To prepare anti-human CD28 monoclonal antibody and analyze its immunological characteristics. Methods BALB / c mice were immunized with the CD28 antigen synthesized by peptide technology. The monoclonal antibodies against CD28 were obtained by the cell hybridization technique. The immunological characteristics of the McAbs were also analyzed. Results After subcloning, 9 anti-human CD28 hybridoma cell lines were successfully obtained, all of which were IgG, 4 were IgG2a and 5 were IgG2b. Five of them were tested for antigenicity by competitive inhibition test, and five were directed against three different antigenic sites respectively. Conclusion The establishment of this McAb cell line is of great significance for further study on the mechanism of action of CD28 McAb on T lymphocytes.