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目的研究胰岛素受体基因第8外显子NsiⅠ位点和过氧化物酶体增殖物激活受体-γ协同刺激因子-1α基因Gly482Ser位点的多态性与空军飞行人员代谢综合征(metabolic syndrome,MS)的相关性,为飞行人员MS预防提供依据。方法采用聚合酶链反应-限制性片段长度多态性技术检测42名MS飞行人员和50名健康对照飞行人员两基因位点的多态性。结果 MS组及健康对照组飞行人员在两基因位点基因型的构成比上均存在显著差异(P<0.05),MS组N2及A等位基因频率显著高于对照组(P<0.05);且N2等位基因对MS的影响大于A等位基因。结论两基因位点的多态性为飞行人员MS的影响因素,N2及A等位基因可能是飞行人员MS相关基因,且N2等位基因与飞行人员的关系较A等位基因密切。
Objective To study the polymorphism of NsiI site of exon 8 of insulin receptor gene and Gly482Ser site of peroxisome proliferator-activated receptor-gamma co-stimulatory factor-1α gene and the risk of metabolic syndrome , MS), provide the basis for prevention of MS in pilots. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphisms of two loci in 42 MS pilots and 50 healthy control pilots. Results There were significant differences in the genotypes between the two gene loci in MS group and healthy control group (P <0.05). The frequencies of N2 and A alleles in MS group were significantly higher than those in control group (P <0.05). And N2 allele of MS greater impact than the A allele. Conclusion The polymorphism of two loci is the influencing factor of flight crew MS. The N2 and A alleles may be the MS related genes of flight crew, and the relationship between N2 allele and flight crew is closer than the A allele.