目的探讨Ⅳ型胶原(CⅣ)、透明质酸(HA)和肿瘤坏死因子α(TNFα)在肝病中的临床意义.方法对94例不同类型肝病应用一步夹心固相酶免疫法检测血清CⅣ活性,并应用放射免疫法检测血清HA和TNFα活性.结果不同类型的肝炎、肝硬变、原发性肝癌(HCC)患者的血清CⅣ,HA和TNFα含量较对照组明显升高(P＜0?05),CⅣ以重症肝炎的含量(μG/L)最高(773±475),HA(μG/L)以硬化型HCC的含量最高(1311±980),TNFα(PMOL/L)以重症肝炎的含量最高(22?5±5?0).血清CⅣ,HA含量与TNFα含量呈正相关.结论血清CⅣ和HA升高反映早期肝纤维化程度,TNFα参与肝纤维化的形成.“,”AIM To study the clinical significance of serum type Ⅳ collagen (C Ⅳ), hyalufonate (HA) and TNFα levels in patients with liver diseases. METHODS C Ⅳ activity was measured using one step Sandwich enzyme immunoassay, and HA and TNFα were measured in 94 patients with liver diseases and 31 controls with radioimmunoassay. RESULTS The serum levels of C Ⅳ, HA and TNFα in various hepatitis, liver cirrhosis and hepatocellular carcinoma (HCC) were higher than those in controls ( P